receptor vectors in pcdna (Addgene inc)
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receptor vectors in pcdna
Receptor Vectors In Pcdna, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 574 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/receptor+vectors+in+pcdna/M50+Super+8x+TOPFlash+(Plasmid+%2312456)/pmc11695057-329-20-33
Average 96 stars, based on 574 article reviews
Receptor Vectors In Pcdna, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 574 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/receptor+vectors+in+pcdna/M50+Super+8x+TOPFlash+(Plasmid+%2312456)/pmc11695057-329-20-33
Average 96 stars, based on 574 article reviews
receptor vectors in pcdna - by Bioz Stars,
2026-09
96/100 stars
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Transfection:Article Title: The co-receptor Tetraspanin12 directly captures Norrin to promote ligand-specific β-catenin signaling Article Snippet: In a six-well plate, cells were seeded at 200,000 cells/well and immediately transfected with 30 picomoles of control siRNA (Sigma SIC001), or 15 picomoles each of two previously validated ( ) Tspan12 siRNA duplexes (#1: 5’- GCUUAUCUUUGCCUUCUCCTT -3’ and 5’-GGAGAAGGCAAAGAUAAGCTT-3’; #2: 5’- AUGAGGGACUACCUAAAUATT -3’ and 5’- UAUUUAGGUAGUCCCUCAUTT -3’) (Sigma) using Lipofectamine RNAiMax (Thermo Fisher) diluted in OptiMEM (Thermo Fisher) according to the manufacturer’s instructions, with media replaced after 16 hr. .. Two days post-transfection, cells were seeded at 12,000 cells/well in white 96-well plates (PerkinElmer) and transfected 4 hr later with Article Title: The co-receptor Tspan12 directly captures Norrin to promote ligand-specific β-catenin signaling Article Snippet: In a 6-well plate, cells were seeded at 200,000 cells per well and immediately transfected with 30 picomoles of control siRNA (Sigma SIC001), or 15 picomoles each of two previously-validated ( ) Tspan12 siRNA duplexes (#1: 5’ -GCUUAUCUUUGCCUUCUCCTT- 3’ and 5’ -GGAGAAGGCAAAGAUAAGCTT- 3’; #2: 5’ -AUGAGGGACUACCUAAAUATT- 3’ and 5’ -UAUUUAGGUAGUCCCUCAUTT- 3’) (Sigma) using Lipofectamine RNAiMax (Thermo Fisher) diluted in OptiMEM (Thermo Fisher) according to manufacturer instructions, with media replaced after 16 hours. .. Two days post-transfection, cells were seeded at 12,000 cells/well in white 96-well plates (PerkinElmer) and transfected 4 hours later with Article Title: The co-receptor Tetraspanin12 directly captures Norrin to promote ligand-specific β-catenin signaling Article Snippet: In a six- well plate, cells were seeded at 200,000 cells/well and immediately transfected with 30 picomoles of control siRNA (Sigma SIC001), or 15 picomoles each of two previously validated (Otomo et al., 2014) Tspan12 siRNA duplexes (#1: 5’- GCUU AUCU UUGC CUUC UCCT T-3’ and 5’- GGAG AAGG CAAA GAUA AGCTT- 3’; #2: 5’- AUGA GGGA CUAC CUAA AUAT T-3’ and 5’- UAUU UAGG UAGU CCCU CAUT T-3’) (Sigma) using Lipofectamine RNAiMax (Thermo Fisher) diluted in OptiMEM (Thermo Fisher) according to the manufacturer’s instructions, with media replaced after 16 hr. .. Two days post- transfection, cells were seeded at 12,000 cells/well in white 96- well plates (PerkinElmer) and transfected 4 hr later with Plasmid Preparation:Article Title: The co-receptor Tetraspanin12 directly captures Norrin to promote ligand-specific β-catenin signaling Article Snippet: In a six-well plate, cells were seeded at 200,000 cells/well and immediately transfected with 30 picomoles of control siRNA (Sigma SIC001), or 15 picomoles each of two previously validated ( ) Tspan12 siRNA duplexes (#1: 5’- GCUUAUCUUUGCCUUCUCCTT -3’ and 5’-GGAGAAGGCAAAGAUAAGCTT-3’; #2: 5’- AUGAGGGACUACCUAAAUATT -3’ and 5’- UAUUUAGGUAGUCCCUCAUTT -3’) (Sigma) using Lipofectamine RNAiMax (Thermo Fisher) diluted in OptiMEM (Thermo Fisher) according to the manufacturer’s instructions, with media replaced after 16 hr. .. Two days post-transfection, cells were seeded at 12,000 cells/well in white 96-well plates (PerkinElmer) and transfected 4 hr later with Article Title: The co-receptor Tspan12 directly captures Norrin to promote ligand-specific β-catenin signaling Article Snippet: In a 6-well plate, cells were seeded at 200,000 cells per well and immediately transfected with 30 picomoles of control siRNA (Sigma SIC001), or 15 picomoles each of two previously-validated ( ) Tspan12 siRNA duplexes (#1: 5’ -GCUUAUCUUUGCCUUCUCCTT- 3’ and 5’ -GGAGAAGGCAAAGAUAAGCTT- 3’; #2: 5’ -AUGAGGGACUACCUAAAUATT- 3’ and 5’ -UAUUUAGGUAGUCCCUCAUTT- 3’) (Sigma) using Lipofectamine RNAiMax (Thermo Fisher) diluted in OptiMEM (Thermo Fisher) according to manufacturer instructions, with media replaced after 16 hours. .. Two days post-transfection, cells were seeded at 12,000 cells/well in white 96-well plates (PerkinElmer) and transfected 4 hours later with Article Title: The co-receptor Tetraspanin12 directly captures Norrin to promote ligand-specific β-catenin signaling Article Snippet: In a six- well plate, cells were seeded at 200,000 cells/well and immediately transfected with 30 picomoles of control siRNA (Sigma SIC001), or 15 picomoles each of two previously validated (Otomo et al., 2014) Tspan12 siRNA duplexes (#1: 5’- GCUU AUCU UUGC CUUC UCCT T-3’ and 5’- GGAG AAGG CAAA GAUA AGCTT- 3’; #2: 5’- AUGA GGGA CUAC CUAA AUAT T-3’ and 5’- UAUU UAGG UAGU CCCU CAUT T-3’) (Sigma) using Lipofectamine RNAiMax (Thermo Fisher) diluted in OptiMEM (Thermo Fisher) according to the manufacturer’s instructions, with media replaced after 16 hr. .. Two days post- transfection, cells were seeded at 12,000 cells/well in white 96- well plates (PerkinElmer) and transfected 4 hr later with |